מעבדה ביולוגית
Semi-dry cell blotter transfer טרנספר חצי יבש לחלבונים
מודל:Trans-Blot SD Semi-Dry Electrophoretic Transfer Cellיצרן:Bio-radמק"ט:170-3940מצב:Pre-owned
Semi-dry cell blotter transfer טרנספר חצי יבש לחלבונים
Western blotting- The technique requires the electrophoretic transfer of proteins, that have been electrophoretically separated on a polyacrylamide gel to nitrocellulose or other types of membrane. The transfer can be performed either in wet (tank) or semi-dry conditions. The Bio-Rad Trans-Blot® SD Semi-Dry Electrophoretic Transfer Cell is a very efficient, highly reproducible blotting system, especially for proteins with low molecular weight.
Western blotting- The technique requires the electrophoretic transfer of proteins, that have been electrophoretically separated on a polyacrylamide gel to nitrocellulose or other types of membrane. The transfer can be performed either in wet (tank) or semi-dry conditions. The Bio-Rad Trans-Blot® SD Semi-Dry Electrophoretic Transfer Cell is a very efficient, highly reproducible blotting system, especially for proteins with low molecular weight.
Western blotting- The technique requires the electrophoretic transfer of proteins, that have been electrophoretically separated on a polyacrylamide gel to nitrocellulose or other types of membrane. The transfer can be performed either in wet (tank) or semi-dry conditions. The Bio-Rad Trans-Blot® SD Semi-Dry Electrophoretic Transfer Cell is a very efficient, highly reproducible blotting system, especially for proteins with low molecular weight.
Western blotting- The technique requires the electrophoretic transfer of proteins, that have been electrophoretically separated on a polyacrylamide gel to nitrocellulose or other types of membrane. The transfer can be performed either in wet (tank) or semi-dry conditions. The Bio-Rad Trans-Blot® SD Semi-Dry Electrophoretic Transfer Cell is a very efficient, highly reproducible blotting system, especially for proteins with low molecular weight.
Turn on the power supply. Transfer mini gels for 15–30 minutes at 10–15 V. Large gels
can be transferred for 30 minutes to 1 hour at 15–25 V. Do not exceed 25 V with this
instrument. A current limit (3 mA/cm2 for large gels; 5.5 mA/cm2 for mini gels) is
recommended to prevent excessive heating during the run. Under the strong fields
developed by this apparatus, transfers may not always be quantitative. A certain quantity
of protein may be transferred through the membrane and onto the filter paper below
Caution: Do not reverse polarity. This will result in damage to the stainless steel cathode
